![]() Further, DMSO showed a significantly higher protective effect against freezing-induced damage than against photo- and γ-ray-irradiation-induced damage. As a result, we found that 2% DMSO conferred the maximum protective effect against all of the injury sources tested, and these effects were maintained at higher concentrations. ![]() In the present study, we aimed to observe the extent of DNA damage by use of single molecular observation with a fluorescence microscope to evaluate DSBs induced by photo- and γ-ray-irradiation, or freeze/thawing in variable concentrations of DMSO. To date, many in vitro assays using cultured cells have been performed for analysing the protective effect of DMSO against genomic DNA damage however, currently it has been rather difficult to detect DNA double strand breaks (DSBs) in a quantitative manner. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. The study showed that the collapsing of DNA structure by the transmutation can be quantitatively evaluated using the root mean square deviation of atomic positions.ĭimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. The effects of tritium transmutation on hydrogen bonds acting between the two main strands of DNA have been examined using molecular dynamics simulations. A new image processing method for the automatic measurement of DNA length using OpenCV and deep learning is also introduced. The factors affecting on the DSB rate are discussed. The effects of β-ray irradiation were not noticeable at the level of tritium concentration of ∼ kBq/cm³, while the irradiation effects were clear at tritium concentrations of ∼ MBq/cm³. The rate of DNA double-strand breaks in tritiated water has been examined using a single molecule observation method. This chapter reviews recent experimental and computer simulation activities on quantitative evaluation of damage rates by β-ray irradiation and nuclear transmutation. Deep knowledges of the mechanisms underlying DNA damages lead to better understanding of biological effects of tritium. DNA molecules are damaged mainly by β-ray irradiation, and additional damages can be induced by break of chemical bond by nuclear transmutation to inert ³He. Tritium is a radioisotope of hydrogen emitting low energy β-rays in disintegration to ³He.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |